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- AG Hauck - LabWiki
- Agarose gel electrophoresis
- All About Safety
- Bacterial Pulldown with soluble receptors
- Bacterial invasion assay by flow cytometry
- Bio-Rad ChemiDoc Touch
- Bio-Rad GelDoc
- Cell Adherence assay
- Cell viability (MTT Assay)
- Cloning of guideRNA-Oligo into pBluescript U6-MCS
- Cloning of guideRNA-Oligo into pLentiCRISPRv2
- Cloning of shRNA into lentiviral vector pLKO.1
- Colony PCR
- Concentration of lentiviral particles via ultracentrifugation
- Coomassie Blue staining of gels
- Covalent coupling of proteins to latex beads
- Cre-Lox recombination of plasmids
- Cultivation of HEK293T cells
- Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing
- Design principles for PCR primers used for amplification and cloning of cDNA
- Designing PCR-Primers for LIC Cloning (Ligation independent cloning)
- Determination of Integrin Activity (ELISA-based)
- Direct labelling of antibodies with fluorescent dyes
- Dot Blot
- Equipment Manuals
- Expression of GST-fusion proteins
- FBA-staining of bacteria
- Fibronectin-Binding Assay
- Flow chart of a PCR-based cloning project
- Fluorescent Staining of Cryosections
- GST-Pulldown
- Generation of competent Pichia pastoris
- Gentamicin Protection Assay
- Granulocyte FACS phagocytosis assay
- Granulocyte oxidative burst
- Immunofluorescence staining
- Immunofluorescence staining of focl adhesion proteins
- Immunoprecipitation
- InFusion-cloning (Fa. Clontech)
- In vitro Tyrosine Kinase Activity Assay
- Infection of granulocytes
- Isolation of DNA from agarose gel
- Isolation of human granulocytes
- LIC Cloning (Ligation independent cloning)
- Lab Etiquette
- Lab Etiquette (ENG)
- Laboratory Bookkeeping Guide
- Laborbuchführung
- Laborsicherheit
- Live cell microscopy
- Luciferase Reporter Assay
- Main Page
- Measurement of endocytosis via reversible surface biotinylation
- Measurement of lipid raft localization via flow cytometry
- Methods and Protocols
- Monitoring bacterial growth
- Neue Seite
- OPTIC (Opa protein triggered integrin clustering)
- Opsonizing bacteria with Fc fusion proteins
- PCR approach in 20 μL with hot-start (qualitative PCR)
- PCR approach in 50 μL with hot-start and/or touch-down (preparative PCR)
- PCR purification
- Plasmid-Miniprep (Birnboim – Dooley protocol)
- Preparation of deterg.-resist. membrane microdomains (Sucrose Gradient)
- Preparation of samples for confocal microscopy
- Production of lentiviral particles
- Production of soluble CEACAM domains in human cells
- Protein Phosphatase Assay
- Protein expression and purification
- Purification of GST-fusion proteins
- Rac pulldown assay (with GST-PBD beads)
- Restriction digest of plasmid DNA
- Rezepte
- S1 - Cell culture guidelines
- SDS-PAGE
- SEAP Reporter Assay
- Scanning electron microscopy (SEM)
- Setting up quantitative real-time PCR qRT-PCR
- SiRNA transfection with INTERFERin
- Site directed mutagenesis
- Staining of cells for flow cytometry
- StrepTactin-Pulldown
- Testexpression in Pichia pastoris
- Thermo Scientific Varioskan Flash
- Thermo Scientific Varioskan Flash eng
- Tissue/embryo preparation fo cryosectioning
- Transduction and titration of lentiviral supernatant
- Transfection of HEK293T cells
- Transfection of fibroblasts
- Transformation of Neisseria gonorrhoeae
- Transformation of Pichia pastoris
- Transformation of plasmids in E. coli
- Viral transduction of mammalian cells via spinfection
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- Vorlage Klonierungsprojekt
- Western Blot: probing of the membrane
- Western Blot: semi-dry blot
- Western Blot: stripping of the membrane
- Western Blot: wet blot
- Whole cell lysates (WCLs) of eukaryotic cells