Coomassie Blue staining of gels
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| Materials | |
|---|---|
| Staining Solution | 25% (v/v) isopropanol
10% (v/v) acetic acid 0.03% (w/v) Coomassie Brillant Blue R250 |
| Destaining Sol. | 500 mL methanol; 500 mL dH2O
100 mL acetic acid |
Procedure
The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a clear background.