Viral transduction of mammalian cells via spinfection

Materials
	Virus containing supernatant
	1000x Polybrene (Hexadimethrine bromide) in PBS (8mg/ml) 1*106 suspension cells or 1*105 adherent cells
	24 well plate (for suspension cells) 6 well plate (for adherent cells)

Procedure

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1. 1 ml medium containing 1*10⁵ or1*10⁶ cells are added into 6 or 24 well plate.
2. Add 2 µl 1000x Polybrene.
3. Add 1ml of virus supernatant or a corresponding dilution of concentrated virus to the well.
4. Perform spinfection at 800 g for 1 h at room temperature, disable the brakes of the centrifuge and spin with acceleration = 1 and deceleration = 1.
5. Change medium after 1-2 days.