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Showing below up to 50 results in range #51 to #100.
- Luciferase Reporter Assay
- Measurement of endocytosis via reversible surface biotinylation
- Measurement of lipid raft localization via flow cytometry
- Methods and Protocols
- Monitoring bacterial growth
- OPTIC (Opa protein triggered integrin clustering)
- Opsonizing bacteria with Fc fusion proteins
- PCR approach in 20 μL with hot-start (qualitative PCR)
- PCR approach in 50 μL with hot-start and/or touch-down (preparative PCR)
- PCR purification
- Plasmid-Miniprep (Birnboim – Dooley protocol)
- Preparation of deterg.-resist. membrane microdomains (Sucrose Gradient)
- Preparation of samples for confocal microscopy
- Production of lentiviral particles
- Production of soluble CEACAM domains in human cells
- Protein Phosphatase Assay
- Protein expression and purification
- Purification of GST-fusion proteins
- Rac pulldown assay (with GST-PBD beads)
- Restriction digest of plasmid DNA
- Rezepte
- S1 - Cell culture guidelines
- SDS-PAGE
- SEAP Reporter Assay
- Scanning electron microscopy (SEM)
- Setting up quantitative real-time PCR qRT-PCR
- SiRNA transfection with INTERFERin
- Site directed mutagenesis
- Staining of cells for flow cytometry
- StrepTactin-Pulldown
- Testexpression in Pichia pastoris
- Thermo Scientific Varioskan Flash
- Thermo Scientific Varioskan Flash eng
- Tissue/embryo preparation fo cryosectioning
- Transduction and titration of lentiviral supernatant
- Transfection of HEK293T cells
- Transfection of fibroblasts
- Transformation of Neisseria gonorrhoeae
- Transformation of Pichia pastoris
- Transformation of plasmids in E. coli
- Viral transduction of mammalian cells via spinfection
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