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  1. AG Hauck - LabWiki
  2. Agarose gel electrophoresis
  3. All About Safety
  4. Bacterial Pulldown with soluble receptors
  5. Bacterial invasion assay by flow cytometry
  6. Bio-Rad ChemiDoc Touch
  7. Bio-Rad GelDoc
  8. Cell Adherence assay
  9. Cell viability (MTT Assay)
  10. Cloning of guideRNA-Oligo into pBluescript U6-MCS
  11. Cloning of guideRNA-Oligo into pLentiCRISPRv2
  12. Cloning of shRNA into lentiviral vector pLKO.1
  13. Colony PCR
  14. Concentration of lentiviral particles via ultracentrifugation
  15. Coomassie Blue staining of gels
  16. Covalent coupling of proteins to latex beads
  17. Cre-Lox recombination of plasmids
  18. Cultivation of HEK293T cells
  19. Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing
  20. Design principles for PCR primers used for amplification and cloning of cDNA
  21. Designing PCR-Primers for LIC Cloning (Ligation independent cloning)
  22. Determination of Integrin Activity (ELISA-based)
  23. Direct labelling of antibodies with fluorescent dyes
  24. Dot Blot
  25. Equipment Manuals
  26. Expression of GST-fusion proteins
  27. FBA-staining of bacteria
  28. Fibronectin-Binding Assay
  29. Flow chart of a PCR-based cloning project
  30. Fluorescent Staining of Cryosections
  31. GST-Pulldown
  32. Generation of competent Pichia pastoris
  33. Gentamicin Protection Assay
  34. Granulocyte FACS phagocytosis assay
  35. Granulocyte oxidative burst
  36. Immunofluorescence staining
  37. Immunofluorescence staining of focl adhesion proteins
  38. Immunoprecipitation
  39. InFusion-cloning (Fa. Clontech)
  40. In vitro Tyrosine Kinase Activity Assay
  41. Infection of granulocytes
  42. Isolation of DNA from agarose gel
  43. Isolation of human granulocytes
  44. LIC Cloning (Ligation independent cloning)
  45. Lab Etiquette
  46. Lab Etiquette (ENG)
  47. Laboratory Bookkeeping Guide
  48. Laborbuchführung
  49. Laborsicherheit
  50. Live cell microscopy

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