Pages with the fewest revisions

Showing below up to 50 results in range #51 to #100.

51. Laborbuchführung (2 revisions)
52. Western Blot: wet blot (2 revisions)
53. Immunofluorescence staining of focl adhesion proteins (2 revisions)
54. Site directed mutagenesis (2 revisions)
55. Western Blot: semi-dry blot (2 revisions)
56. Luciferase Reporter Assay (2 revisions)
57. Testexpression in Pichia pastoris (2 revisions)
58. Cultivation of HEK293T cells (2 revisions)
59. Thermo Scientific Varioskan Flash (2 revisions)
60. Western Blot: stripping of the membrane (2 revisions)
61. Expression of GST-fusion proteins (2 revisions)
62. Granulocyte FACS phagocytosis assay (2 revisions)
63. Transformation of plasmids in E. coli (2 revisions)
64. Staining of cells for flow cytometry (2 revisions)
65. StrepTactin-Pulldown (2 revisions)
66. Gentamicin Protection Assay (2 revisions)
67. Measurement of endocytosis via reversible surface biotinylation (2 revisions)
68. Laboratory Bookkeeping Guide (2 revisions)
69. Concentration of lentiviral particles via ultracentrifugation (2 revisions)
70. Transfection of HEK293T cells (2 revisions)
71. Isolation of DNA from agarose gel (2 revisions)
72. Viral transduction of mammalian cells via spinfection (2 revisions)
73. Flow chart of a PCR-based cloning project (2 revisions)
74. PCR purification (2 revisions)
75. Plasmid-Miniprep (Birnboim – Dooley protocol) (2 revisions)
76. LIC Cloning (Ligation independent cloning) (3 revisions)
77. Generation of competent Pichia pastoris (3 revisions)
78. Vorlage:Note (3 revisions)
79. Monitoring bacterial growth (3 revisions)
80. PCR approach in 20 μL with hot-start (qualitative PCR) (3 revisions)
81. Cloning of guideRNA-Oligo into pBluescript U6-MCS (3 revisions)
82. Production of soluble CEACAM domains in human cells (3 revisions)
83. Cre-Lox recombination of plasmids (3 revisions)
84. OPTIC (Opa protein triggered integrin clustering) (3 revisions)
85. Laborsicherheit (3 revisions)
86. Cloning of guideRNA-Oligo into pLentiCRISPRv2 (3 revisions)
87. Immunoprecipitation (3 revisions)
88. Cloning of shRNA into lentiviral vector pLKO.1 (3 revisions)
89. Transformation of Pichia pastoris (4 revisions)
90. Colony PCR (4 revisions)
91. Setting up quantitative real-time PCR qRT-PCR (5 revisions)
92. Transfection of fibroblasts (5 revisions)
93. Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing (5 revisions)
94. Agarose gel electrophoresis (6 revisions)
95. Purification of GST-fusion proteins (6 revisions)
96. Equipment Manuals (6 revisions)
97. Designing PCR-Primers for LIC Cloning (Ligation independent cloning) (6 revisions)
98. S1 - Cell culture guidelines (7 revisions)
99. Lab Etiquette (ENG) (10 revisions)
100. All About Safety (12 revisions)