Pages with the fewest revisions

Showing below up to 50 results in range #51 to #100.

51. Western Blot: wet blot (2 revisions)
52. Immunofluorescence staining of focl adhesion proteins (2 revisions)
53. Western Blot: semi-dry blot (2 revisions)
54. Site directed mutagenesis (2 revisions)
55. Testexpression in Pichia pastoris (2 revisions)
56. Luciferase Reporter Assay (2 revisions)
57. Cultivation of HEK293T cells (2 revisions)
58. Western Blot: stripping of the membrane (2 revisions)
59. Thermo Scientific Varioskan Flash (2 revisions)
60. Transformation of plasmids in E. coli (2 revisions)
61. Expression of GST-fusion proteins (2 revisions)
62. Granulocyte FACS phagocytosis assay (2 revisions)
63. Staining of cells for flow cytometry (2 revisions)
64. StrepTactin-Pulldown (2 revisions)
65. Measurement of endocytosis via reversible surface biotinylation (2 revisions)
66. Gentamicin Protection Assay (2 revisions)
67. Concentration of lentiviral particles via ultracentrifugation (2 revisions)
68. Transfection of HEK293T cells (2 revisions)
69. Isolation of DNA from agarose gel (2 revisions)
70. Laboratory Bookkeeping Guide (2 revisions)
71. Viral transduction of mammalian cells via spinfection (2 revisions)
72. PCR purification (2 revisions)
73. Plasmid-Miniprep (Birnboim – Dooley protocol) (2 revisions)
74. Flow chart of a PCR-based cloning project (2 revisions)
75. Laborbuchführung (2 revisions)
76. LIC Cloning (Ligation independent cloning) (3 revisions)
77. Generation of competent Pichia pastoris (3 revisions)
78. Vorlage:Note (3 revisions)
79. Monitoring bacterial growth (3 revisions)
80. Production of soluble CEACAM domains in human cells (3 revisions)
81. Cre-Lox recombination of plasmids (3 revisions)
82. PCR approach in 20 μL with hot-start (qualitative PCR) (3 revisions)
83. Cloning of guideRNA-Oligo into pBluescript U6-MCS (3 revisions)
84. OPTIC (Opa protein triggered integrin clustering) (3 revisions)
85. Laborsicherheit (3 revisions)
86. Cloning of guideRNA-Oligo into pLentiCRISPRv2 (3 revisions)
87. Immunoprecipitation (3 revisions)
88. Cloning of shRNA into lentiviral vector pLKO.1 (3 revisions)
89. Transformation of Pichia pastoris (4 revisions)
90. Colony PCR (4 revisions)
91. Setting up quantitative real-time PCR qRT-PCR (5 revisions)
92. Transfection of fibroblasts (5 revisions)
93. Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing (5 revisions)
94. Purification of GST-fusion proteins (6 revisions)
95. Equipment Manuals (6 revisions)
96. Designing PCR-Primers for LIC Cloning (Ligation independent cloning) (6 revisions)
97. Agarose gel electrophoresis (6 revisions)
98. S1 - Cell culture guidelines (7 revisions)
99. Lab Etiquette (ENG) (10 revisions)
100. All About Safety (12 revisions)