Pages with the most revisions

Showing below up to 50 results in range #1 to #50.

1. AG Hauck - LabWiki (29 revisions)
2. Methods and Protocols (26 revisions)
3. Lab Etiquette (22 revisions)
4. PCR approach in 50 μL with hot-start and/or touch-down (preparative PCR) (13 revisions)
5. Protein expression and purification (12 revisions)
6. All About Safety (12 revisions)
7. Lab Etiquette (ENG) (10 revisions)
8. S1 - Cell culture guidelines (7 revisions)
9. Equipment Manuals (6 revisions)
10. Designing PCR-Primers for LIC Cloning (Ligation independent cloning) (6 revisions)
11. Agarose gel electrophoresis (6 revisions)
12. Purification of GST-fusion proteins (6 revisions)
13. Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing (5 revisions)
14. Setting up quantitative real-time PCR qRT-PCR (5 revisions)
15. Transfection of fibroblasts (5 revisions)
16. Colony PCR (4 revisions)
17. Transformation of Pichia pastoris (4 revisions)
18. OPTIC (Opa protein triggered integrin clustering) (3 revisions)
19. Laborsicherheit (3 revisions)
20. Cloning of guideRNA-Oligo into pLentiCRISPRv2 (3 revisions)
21. Immunoprecipitation (3 revisions)
22. Cloning of shRNA into lentiviral vector pLKO.1 (3 revisions)
23. LIC Cloning (Ligation independent cloning) (3 revisions)
24. Generation of competent Pichia pastoris (3 revisions)
25. Vorlage:Note (3 revisions)
26. Monitoring bacterial growth (3 revisions)
27. Production of soluble CEACAM domains in human cells (3 revisions)
28. Cre-Lox recombination of plasmids (3 revisions)
29. PCR approach in 20 μL with hot-start (qualitative PCR) (3 revisions)
30. Cloning of guideRNA-Oligo into pBluescript U6-MCS (3 revisions)
31. Transformation of plasmids in E. coli (2 revisions)
32. Expression of GST-fusion proteins (2 revisions)
33. Granulocyte FACS phagocytosis assay (2 revisions)
34. Staining of cells for flow cytometry (2 revisions)
35. StrepTactin-Pulldown (2 revisions)
36. Measurement of endocytosis via reversible surface biotinylation (2 revisions)
37. Gentamicin Protection Assay (2 revisions)
38. Transfection of HEK293T cells (2 revisions)
39. Isolation of DNA from agarose gel (2 revisions)
40. Laboratory Bookkeeping Guide (2 revisions)
41. Concentration of lentiviral particles via ultracentrifugation (2 revisions)
42. Viral transduction of mammalian cells via spinfection (2 revisions)
43. PCR purification (2 revisions)
44. Plasmid-Miniprep (Birnboim – Dooley protocol) (2 revisions)
45. Flow chart of a PCR-based cloning project (2 revisions)
46. Laborbuchführung (2 revisions)
47. Western Blot: wet blot (2 revisions)
48. Immunofluorescence staining of focl adhesion proteins (2 revisions)
49. Western Blot: semi-dry blot (2 revisions)
50. Site directed mutagenesis (2 revisions)