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Showing below up to 50 results in range #1 to #50.

1. Agarose gel electrophoresis
2. Bacterial Pulldown with soluble receptors
3. Bacterial invasion assay by flow cytometry
4. Bio-Rad ChemiDoc Touch
5. Bio-Rad GelDoc
6. Cell Adherence assay
7. Cell viability (MTT Assay)
8. Cloning of guideRNA-Oligo into pBluescript U6-MCS
9. Cloning of guideRNA-Oligo into pLentiCRISPRv2
10. Cloning of shRNA into lentiviral vector pLKO.1
11. Colony PCR
12. Concentration of lentiviral particles via ultracentrifugation
13. Coomassie Blue staining of gels
14. Covalent coupling of proteins to latex beads
15. Cre-Lox recombination of plasmids
16. Cultivation of HEK293T cells
17. Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing
18. Design principles for PCR primers used for amplification and cloning of cDNA
19. Designing PCR-Primers for LIC Cloning (Ligation independent cloning)
20. Determination of Integrin Activity (ELISA-based)
21. Direct labelling of antibodies with fluorescent dyes
22. Dot Blot
23. Equipment Manuals
24. Expression of GST-fusion proteins
25. FBA-staining of bacteria
26. Fibronectin-Binding Assay
27. Fluorescent Staining of Cryosections
28. GST-Pulldown
29. Generation of competent Pichia pastoris
30. Gentamicin Protection Assay
31. Granulocyte FACS phagocytosis assay
32. Granulocyte oxidative burst
33. Immunofluorescence staining
34. Immunofluorescence staining of focl adhesion proteins
35. Immunoprecipitation
36. InFusion-cloning (Fa. Clontech)
37. In vitro Tyrosine Kinase Activity Assay
38. Infection of granulocytes
39. Isolation of DNA from agarose gel
40. Isolation of human granulocytes
41. LIC Cloning (Ligation independent cloning)
42. Live cell microscopy
43. Luciferase Reporter Assay
44. Measurement of endocytosis via reversible surface biotinylation
45. Measurement of lipid raft localization via flow cytometry
46. Monitoring bacterial growth
47. OPTIC (Opa protein triggered integrin clustering)
48. Opsonizing bacteria with Fc fusion proteins
49. PCR approach in 20 μL with hot-start (qualitative PCR)
50. PCR approach in 50 μL with hot-start and/or touch-down (preparative PCR)