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  • 15:21, 30 May 2025 ESO wikiadmin talk contribs uploaded File:Protokoll guideRNA.jpg
  • 15:18, 30 May 2025 ESO wikiadmin talk contribs created page Design of guideRNA-Oligos (gRNA) for CRISPR/Cas gen. editing (Created page with "{{Protokoll-Mix|Inhalt= 20 mM dNTPs<br>10x Taq/Pfu buffer<br>2 Primers 10 μM<br> DNA template, 100 – 200 ng/μL<br>DNA polymerases (Pfu, Taq)}}") Tag: Visual edit: Switched
  • 12:13, 30 May 2025 ESO wikiadmin talk contribs created page Cloning of shRNA into lentiviral vector pLKO.1 (Created page with "== Procedure == === Primer annealing === * annealing reaction contains: 1 µl sense primer (stock solution 100 µM), 1 µl antisense primer (stock solution 100 µM), 5 µl NEB buffer 2 and 43 µl ddH<sub>2</sub>O * Annealing reaction occurs in thermocycler with the corresponding program:") Tag: Visual edit
  • 12:04, 30 May 2025 ESO wikiadmin talk contribs created page LIC Cloning (Ligation independent cloning) (Created page with " == Procedure == Design primers compatible for cloning in vector containing the LIC sequence (e.g. pDNR Dual LIC) and perform PCR. Separate 4 µl of PCR reaction via agarose gel electrophoresis to check PCR product. Many unspecific bands besides the desired one require gel separation of the PCR sample and extraction of the desired band. If necessary, 2-3 PCR reactions can be pooled to gain more material. Add 10x NEB2 Buffer to the PCR sample to reach 1x end concentration...") Tag: Visual edit: Switched
  • 11:48, 30 May 2025 ESO wikiadmin talk contribs created page File:LIC sequences.jpg (Fig. 1: Sequences to include in forward and reverse primers intended for LIC cloning. Codons representing the proper reading frame after transfer from pDNR-dual LIC are indicated. NNN indicates the start of the sequence complementary to your target sequence. Please remem- ber to use the antisense sequence in the reverse primer.)
  • 11:48, 30 May 2025 ESO wikiadmin talk contribs uploaded File:LIC sequences.jpg (Fig. 1: Sequences to include in forward and reverse primers intended for LIC cloning. Codons representing the proper reading frame after transfer from pDNR-dual LIC are indicated. NNN indicates the start of the sequence complementary to your target sequence. Please remem- ber to use the antisense sequence in the reverse primer.)
  • 11:29, 30 May 2025 ESO wikiadmin talk contribs created page Template:Abbildung (Created page with "<div style="text-align: center; margin: 25px 0; padding: 15px; border: 1px solid #a2a9b1; border-radius: 4px; background: #f8f9fa;"> [[Datei:{{{datei}}}|{{{groesse|400px}}}]] <div style="margin-top: 10px; font-size: 13px; color: #555; line-height: 1.4;"> <strong>{{{titel|Fig. 1:}}}</strong> {{{beschreibung|Bildbeschreibung}}} </div> </div>")
  • 11:28, 30 May 2025 ESO wikiadmin talk contribs created page Designing PCR-Primers for LIC Cloning (Ligation independent cloning) (Created page with " == Procedure == Delineate the coding sequence (full-length or partial sequence), which you want to amplify. Sense and antisense primers should have around 18 to 22 complementary bases, depending on the AT-content (there should be 9-10 Gs or Cs in the primer- cDNA complementary region). Both primers should ideally end with a 3’-GC clamp (two G or C bases). In the antisense primer, add a STOP codon, if you want to create a protein with an N- terminal tag; omit the STOP...") Tag: Visual edit
  • 11:23, 30 May 2025 ESO wikiadmin talk contribs created page Isolation of DNA from agarose gel (Created page with "{{Protokoll-Mix|Inhalt= 20 mM dNTPs<br>10x Taq/Pfu buffer<br>2 Primers 10 μM<br> DNA template, 100 – 200 ng/μL<br>DNA polymerases (Pfu, Taq)}}") Tag: Visual edit: Switched
  • 11:21, 30 May 2025 ESO wikiadmin talk contribs created page PCR purification (Created page with "{{Protokoll-Mix|Inhalt=TAE buffer (1x) 4.84 g Tris-Base, 1 mM EDTA, 1.14 mL acetic acid, fill 1 L with A.bidest. Agarose}}") Tag: Visual edit: Switched
  • 10:00, 30 May 2025 ESO wikiadmin talk contribs created page Setting up quantitative real-time PCR qRT-PCR (Created page with "{{Pipetten-Checklist-8step}}") Tag: Visual edit
  • 09:59, 30 May 2025 ESO wikiadmin talk contribs created page Template:Pipetten-Checklist-8step (Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> <div>✓ {{{titel|Pipettierschema - Checklist}}}</div> </div> <div style="padding: 15px; color: #333; font-size: 13px;"> <div> <table...")
  • 09:41, 30 May 2025 ESO wikiadmin talk contribs created page PCR approach in 20 μL with hot-start (qualitative PCR) (Created page with "{{Protokoll-Mix|Inhalt= The same as for PCR approach in 50 µL}}")
  • 09:33, 30 May 2025 ESO wikiadmin talk contribs created page Template:Pipetten-Checklist-flexibel (Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> <div>✓ {{{titel|Pipettierschema - Checklist}}}</div> </div> <div style="padding: 15px; color: #333; font-size: 13px;"> <div> <table...")
  • 18:18, 29 May 2025 ESO wikiadmin talk contribs created page Template:Pipetten-Checklist (Pipettierschmema allgemein)
  • 18:16, 29 May 2025 ESO wikiadmin talk contribs created page Template:Pipettierschema (Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> <div>✓ {{{titel|Pipettierschema - Checklist}}}</div> </div> <div style="padding: 15px; color: #333; font-size: 13px;"> <div> <table...")
  • 18:12, 29 May 2025 ESO wikiadmin talk contribs created page PCR approach in 50 μL with hot-start and/or touch-down (preparative PCR) (Created page with "{{Protokoll-Mix|Inhalt=20 mM dNTPs 10x Taq/Pfu buffer 2 Primers, 10 μM DNA template, 100 – 200 ng/μL DNA polymerases (Pfu, Taq)}} == Procedure == As usually more than one PCR sample is run simultaneously, reaction and enzyme mix are prepared separately. The enzyme mix is added to the reaction mix after starting the PCR and after pre-heating the reaction mix to 94 ºC (hot start). A typical 50 μL approach that yields enough material for later cloning procedures...") Tag: Visual edit
  • 18:04, 29 May 2025 ESO wikiadmin talk contribs created page Design principles for PCR primers used for amplification and cloning of cDNA (Created page with "'''Restriction Enzyme Sites:''' For conventional cloning with restriction enzymes, the recognition sites are added to the 5’ end of the primer. Check that the involved '''restriction enzymes do NOT cut within the cloned cDNA''' and that they do '''NOT cut the vector outside the intended position'''. Make sure to preserve the reading frame, if the DNA is cloned 3’ or 5’ of existing ORFs (e.g. GFP) in the vector. Add 3 to 4 meaningless nucleotides (e.g. ATAA-) at the...") Tag: Visual edit
  • 17:56, 29 May 2025 ESO wikiadmin talk contribs created page Agarose gel electrophoresis (Created page with "{{Protokoll-Mix |titel=PCR Reaction mix (Pipett scheme) |TAE buffer (1x) 4.84 g Tris-Base, 1 mM EDTA, 1.14 mL acetic acid, fill 1 L with A.bidest. Agarose }}")
  • 17:54, 29 May 2025 ESO wikiadmin talk contribs created page Template:Protokoll-Mix (Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> <div>{{{titel|Reaction mix}}}</div> </div> <div style="padding: 12px 15px; color: #333; font-size: 13px; font-family: monospace;"> <div> {{{...")
  • 17:31, 29 May 2025 ESO wikiadmin talk contribs created page Template:Minimal-Box (Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> {{{titel|Titel}}} </div> <div style="padding: 12px 15px; color: #333; font-size: 13px;"> {{{inhalt|Inhalt der Box}}} </div> </div>")
  • 16:42, 29 May 2025 ESO wikiadmin talk contribs created page Template:Protokoll-Box (Created page with "<div style="border: 1px solid #ddd; border-radius: 8px; padding: 20px; margin: 20px 0; background: #fff;"> <h2 style="color: #0645ad; border-bottom: 2px solid #0645ad; padding-bottom: 10px;"> 🧪 {{{titel|Laborprotokoll}}} </h2> <div style="display: grid; grid-template-columns: 2fr 1fr; gap: 20px; margin-top: 15px;"> <!-- Hauptinhalt --> <div> <h3>Beschreibung</h3> <p>{{{beschreibung|Protokollbeschreibung hier}}}</p> <h3>Mat...")
  • 16:42, 29 May 2025 ESO wikiadmin talk contribs created page Template:File-Preview (Created page with "<div style="border: 1px solid #ddd; border-radius: 4px; padding: 10px; margin: 10px 0; background: #f9f9f9; display: inline-block; width: 200px;"> <div style="text-align: center; margin-bottom: 8px;"> <div style="width: 64px; height: 64px; margin: 0 auto; background: {{{color|#d73027}}}; border-radius: 4px; display: flex; align-items: center; justify-content: center; color: white; font-weight: bold; font-size: 18px;"> {{{icon|📄}}} </div> </div> <di...")
  • 16:24, 29 May 2025 ESO wikiadmin talk contribs created page Template:Labor-Boxes (Created page with "<div style="display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin: 20px 0;"> <!-- Box 1 --> <div style="background: #e8f4fd; border: 1px solid #3366cc; border-radius: 4px; padding: 16px; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <h3 style="margin-top: 0; color: #0645ad; border-bottom: 1px solid #3366cc; padding-bottom: 8px; margin-bottom: 12px;">🧪 Protokolle</h3> <div>Dokumentation aller Laborexperimente und Versuchsabläufe.</div> <div st...")
  • 16:21, 29 May 2025 ESO wikiadmin talk contribs created page Template:Homepage-Grid (Created page with "<div style="display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin: 20px 0;"> <div style="grid-column: 1;"> {{Colored-Box |title={{{title1|Titel 1}}} |content={{{content1|Inhalt Box 1}}} |background={{{bg1|#e8f4fd}}} |border={{{border1|#3366cc}}} |link={{{link1|}}} |link-text={{{linktext1|Mehr →}}} }} </div> <div style="grid-column: 2;"> {{Colored-Box |title={{{title2|Titel 2}}} |content={{{content2|Inhalt Box...")
  • 16:20, 29 May 2025 ESO wikiadmin talk contribs created page Template:Colored-Box (Created page with "<div class="colored-box" style="background: {{{background|#f8f9fa}}}; border: 1px solid {{{border|#a2a9b1}}}; border-radius: 4px; padding: 16px; margin: 10px 0; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> {{#if:{{{title|}}}|<h3 style="margin-top: 0; color: {{{title-color|#0645ad}}}; border-bottom: 1px solid {{{border|#a2a9b1}}}; padding-bottom: 8px; margin-bottom: 12px;">{{{title}}}</h3>}} <div style="color: {{{text-color|#333}}};"> {{{content|Box-Inhalt hier}}} <...")
  • 16:18, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Homepage-Grid (Created page with "<div style="display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin: 20px 0;"> <div style="grid-column: 1;"> {{Colored-Box |title={{{title1|Titel 1}}} |content={{{content1|Inhalt Box 1}}} |background={{{bg1|#e8f4fd}}} |border={{{border1|#3366cc}}} |link={{{link1|}}} |link-text={{{linktext1|Mehr →}}} }} </div> <div style="grid-column: 2;"> {{Colored-Box |title={{{title2|Titel 2}}} |content={{{content2|Inhalt Box...")
  • 16:18, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Colored-Box (Created page with "<div class="colored-box" style="background: {{{background|#f8f9fa}}}; border: 1px solid {{{border|#a2a9b1}}}; border-radius: 4px; padding: 16px; margin: 10px 0; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> {{#if:{{{title|}}}|<h3 style="margin-top: 0; color: {{{title-color|#0645ad}}}; border-bottom: 1px solid {{{border|#a2a9b1}}}; padding-bottom: 8px; margin-bottom: 12px;">{{{title}}}</h3>}} <div style="color: {{{text-color|#333}}};"> {{{content|Box-Inhalt hier}}} <...")
  • 16:15, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Tip (Created page with "<div style="border-left: 4px solid #8b5cf6; background: #f5f3ff; padding: 12px; margin: 16px 0; border-radius: 2px;"> <strong>💡 Tipp:</strong> {{{1|Tipp-Text}}} </div>")
  • 16:14, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Info (Created page with "<div style="border-left: 4px solid #00af89; background: #f0fdf4; padding: 12px; margin: 16px 0; border-radius: 2px;"> <strong>ℹ️ Info:</strong> {{{1|Informationstext}}} </div>")
  • 16:14, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Warning (Created page with "<div style="border-left: 4px solid #d73027; background: #fdf2f2; padding: 12px; margin: 16px 0; border-radius: 2px;"> <strong>⚠️ Warning:</strong> {{{1|Warntext}}} </div>")
  • 16:09, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Note (Hinweisbox)
  • 15:47, 29 May 2025 ESO wikiadmin talk contribs created page Template:Print-Button (Created page with "<div class="print-export-buttons" style="float: right; margin: 10px 0; padding: 10px; border: 1px solid #ddd; background: #f8f9fa; border-radius: 3px;"> <strong>📄 Export:</strong><br> <button onclick="window.print()" style="padding: 5px 10px; margin: 2px; background: #0645ad; color: white; border: none; cursor: pointer; border-radius: 3px;"> 🖨️ Drucken/PDF </button><br> <button onclick="copyContent()" style="padding: 5px 10px; margin: 2px; background:...")
  • 15:44, 29 May 2025 ESO wikiadmin talk contribs created page Vorlage:Print-Button (Print/Export-Button-Vorlage erstellt)
  • 15:10, 29 May 2025 ESO wikiadmin talk contribs created page Colony PCR (Created page with "Reaction mix: H<sub>2</sub>O                                         16.5 μL 10x Taq/Pfu buffer                       2 μL Primer 1                                    0.4 μL (10 pmol) Primer 2                                    0.4 μL (10 pmol) dNTP-Mix                                  0.2 μL Taq polymerase                        0.5 μL total              ...") Tag: Visual edit
  • 14:14, 21 May 2025 ESO wikiadmin talk contribs created page Restriction digest of plasmid DNA (Created page with "{| class="wikitable" |+ !Materials |- |Restriction enzyme stock (–20°C) |- |Appropriate 10x enzyme buffer (–20°C) |- |Sterile dd H<sub>2</sub>O |- |Depending on the digestion '''(optional)''': '''''BSA (bovine serum albumin)''''': To ensure 100% activity of the enzymes, 100 µg/ml BSA should be added (stored at –20°C) |} == Procedure for analysis purposes == * 300 ng of DNA samples (e.g. control digest of plasmid minipreps) * 1 µl appropriate enzyme buffer (1...") Tag: Visual edit
  • 14:11, 21 May 2025 ESO wikiadmin talk contribs created page Plasmid-Miniprep (Birnboim – Dooley protocol) (Created page with "{| class="wikitable" |+ !Materials |- |Buffer P1: Solve 6.055 g Tris Base and 3.722g EDTA x 2H<sub>2</sub>O in 800 ml H<sub>2</sub>0. Adjust pH to 8.0 with 1M HCl, fill up to 1L. Per 1 ml buffer P1, add 100 µg RNase A. Store at 4°C. |- |Buffer P2: Solve 8.0 g NaOH in 950 ml H<sub>2</sub>0. Add 50 ml 20% SDS. Store at RT. |- |Puffer P3: 294.45g KAc in 500 ml H<sub>2</sub>O. Adjust pH to 5.5 with glacial acetic acid (about 110 ml acid needed), fill up to 1L with H<sub>...") Tag: Visual edit
  • 15:27, 20 May 2025 ESO wikiadmin talk contribs created page Vorlage Klonierungsprojekt (Created page with "<noinclude> == Vorlage für Klonierungsprojekte == Diese Seite dient als Vorlage für neue gentechnische Arbeiten im Labor (z. B. PCR-Klonierungen). Kopiere den folgenden Inhalt in eine neue Wiki-Seite und passe ihn projektbezogen an. '''Hinweis:''' Alle Datumsangaben, Enzyme, Vektoren etc. bitte entsprechend aktualisieren.</noinclude> == Projekttitel == ''(z. B. Klonierung von GFP-tagged PAK1 in pcDNA3.1)'' * Startdatum: __.__.20__ * Bearbeiter: Benutzer:DeinN...") Tag: Visual edit
  • 15:16, 20 May 2025 ESO wikiadmin talk contribs created page Flow chart of a PCR-based cloning project (Created page with "== Experimenteller Ablaufplan eines klassischen PCR-basierten Klonierungsprojekts == ''(LIC-basiertes Klonieren kann in vergleichbarer Zeit mit ähnlichen Schritten durchgeführt werden)'' === Montag === * PCR (5 × 50 µl Reaktionen im Temperaturgradienten) * 5 µl jeder PCR-Reaktion auf Agarosegel laden, Produktgröße & Qualität kontrollieren * 2–3 Proben kombinieren (3 × 45 µl), 5 µl DpnI hinzufügen, 1 h bei 37 °C inkubieren * 5-faches Volumen Puffer PB (...") Tag: Visual edit: Switched
  • 15:09, 20 May 2025 ESO wikiadmin talk contribs created page Fluorescent Staining of Cryosections (Created page with "{| class="wikitable" |+ !Materials |- |PBS pH 7.4 |- |Blocking buffer (1% BSA, 1% serum and 0.1% Triton X-100 in PBS) |- |Corresponding 1st and 2nd antibodies |- |Barrier pen |- |Mounting medium |} === Procedure: === * Thaw the slides at room temperature for 10-20 min * The slides are washed for 10 min in PBS * Block non-specific staining by incubating samples in blocking buffer for 30 minutes at RT * Surround the tissue with a hydrophobic barrier using a barrier pen...") Tag: Visual edit
  • 15:06, 20 May 2025 ESO wikiadmin talk contribs created page Tissue/embryo preparation fo cryosectioning (Created page with "{| class="wikitable" |+ !Materials |- |PBS pH 7.4 |- |4% PFA in PBS |- |10%, 20% and 30% sucrose solution in PBS |- |OCT Tissue-Tek |- |Histology moulds |- |Pre-coated glass slides |- |Dry ice |} === Procedure: === * Tissues/ anesthetized embryos are dissected and placed into 4% PFA solution overnight at 4° C * The next day the specimen is washed with PBS and transferred to a 10% sucrose solution for 3 h at 4° C (keep at 4°C until specimen sinks) * The sample is tra...") Tag: Visual edit
  • 15:03, 20 May 2025 ESO wikiadmin talk contribs created page Bacterial invasion assay by flow cytometry (Created page with "{| class="wikitable" |+ !Materials (for 6 cm dishes) |- |CFSE |- |FACS buffer (1% (F)CS in PBS, 50-100 mL (ice-cold) |- |Trypsin/EDTA (1mL/dish) |- |Medium with serum according to cell type (3mL/dish) |- |15 mL PP falcon tubes |- |Trypan blue solution, 0.4 mg/mL |- |Eppendorf tubes 1.5mL (1 per dish) |} === Procedure === Bacteria: * 2 days before the experiment, streak bacteria from glycerol stock on selective GC or TSB agar plates (with antibiotics when needed) and in...") Tag: Visual edit
  • 09:57, 20 May 2025 ESO wikiadmin talk contribs created page Staining of cells for flow cytometry (Created page with "{| class="wikitable" |+ ! ! ! ! |- |FACS buffer: |1xPBS FCS (heat-inactivated, final concentration: 5%) 5% Natriumazid (final concentration: 0.1%) | | |- |1st antibody: |primary antibody (approx. dilution 1:200) | | |- |2nd antibody: |secondary antibody (approx. dilution 1:500) fluorescence- labelled | | |- |controls: |sample with irrelevant primary antibody and secondary antibody sample with secondary antibody only if possible untransfected cells stained with both antib...") Tag: Visual edit
  • 09:54, 20 May 2025 ESO wikiadmin talk contribs created page Scanning electron microscopy (SEM) (Created page with "{| class="wikitable" |+ !Materials |- |'''Fixation buffer''': 3% Formaldehyde, 2% Glutardialdehyde, 10 mM CaCl<sub>2</sub>, 10 mM MgCl<sub>2</sub>, 90 mM Sucrose in 100 mM HEPES, pH 7.2 |- |'''Washing Buffer''': 10 mM CaCl<sub>2</sub>, 10 mM MgCl<sub>2</sub>, 90 mM Sucrose in 100 mM HEPES, pH 7.2 |- |'''HEPES buffer:''' 100 mM, pH 7.2 |- |'''Graded ethanol series:''' 30%, 50%, 70%, 90%, 100% ethanol p.a. |} === Procedure: === '''1A. ''Organs''''' of infected animals are...") Tag: Visual edit
  • 09:43, 20 May 2025 ESO wikiadmin talk contribs created page OPTIC (Opa protein triggered integrin clustering) (Created page with "{| class="wikitable" |+ !Materials |- |1x PBS |- |Trypsin/EDTA |- |GC-Agar plates with CAM/ERM and without antibiotics Coverslips (round, 12 mm) |- |24 well plate |- |HEK culture medium (DMEM + 10% CS) Suspension medium (DMEM + 0.25% BSA) 4% paraformaldehyde (PFA) in PBS |- |Permeabilization solution (0.4% Triton-X-100 in PBS) Blocking solution (10% heat inactivated CS in PBS) Mounting medium |} === Procedure === ==== '''Day1:''' ==== ==== Seed HEK293T cells 1:40 in 6...") Tag: Visual edit
  • 09:39, 20 May 2025 ESO wikiadmin talk contribs created page Live cell microscopy (Created page with "=== Preparation of culture dishes === * In 3.5 cm culture dishes (Sarstedt 83.1800 35x10 mm) a hole is drilled with 14 mm in diameter (will be done by the uni garage) * Before sticking the coverslips (Menzel 24 mm, Stärke 1,5; CB00240RAC20MNZ#0) to the dishes spray them with 70% ethanol and wipe with a KimWipe tissue * The coverslips are pretreated as following: ** Wash with a mixture of 50% v/v Aceton and 50% v/v EtOH ** Wash with 1xPBS ** Wash with MilliQ water ** Dr...") Tag: Visual edit
  • 15:45, 7 May 2025 ESO wikiadmin talk contribs created page Lab Etiquette (ENG) (Created page with "'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;"> '''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>''' A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let p...")
  • 15:28, 7 May 2025 ESO wikiadmin talk contribs created page Laborbuchführung (Created page with "'''Diese Seite ist auch auf Englisch verfügbar.''' = Laboratory Bookkeeping Guide = == Ein praxisorientierter Leitfaden für sauberes und effizientes Arbeiten im Forschungslabor == === Warum Laborbuchführung wichtig ist === Saubere Buchführung im Labor ist kein lästiger Formalismus – sie ist ein Grundpfeiler wissenschaftlicher Integrität. [...] === Grundprinzipien === # '''Nachvollziehbarkeit''' # '''Unveränderbarkeit''' # '''Z...")
  • 15:27, 7 May 2025 ESO wikiadmin talk contribs created page Laboratory Bookkeeping Guide (Created page with "'''This page is also available in German.''' = Laboratory Bookkeeping Guide = == A practical guide to structured and efficient lab documentation == === Why lab bookkeeping matters === Clean lab documentation isn’t just bureaucracy – it’s a cornerstone of scientific integrity. [...] === Core principles === # '''Traceability''' # '''Immutability''' # '''Timeliness''' # '''Structure''' === Analog or digital? === [...] === What to include =...") Tag: Visual edit
  • 10:49, 7 March 2025 ESO wikiadmin talk contribs created page Immunofluorescence staining of focl adhesion proteins (Created page with "Materials PBS++ (1x PBS + 0.9mM CaCl<sub>2</sub> + 0.5 mM MgCl<sub>2</sub>) Coverslips (round, 12 mm) 24 well plate 4% paraformaldehyde (PFA) in PBS Permeabilization/fixation solution (4% PFA/PBS + 0.1% Triton-X-100) Blocking solution (10% heat inactivated CS in PBS) Mounting medium {| class="wikitable" |+ !Materials ! |- | |PBS++ (1x PBS + 0.9mM CaCl<sub>2</sub> + 0.5 mM MgCl<sub>2</sub>) |- | |Coverslips (round, 12 mm) |- | |24 well plate |- | |4% paraformaldehyd...") Tag: Visual edit
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