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• 18:50, 15 February 2025 ESO wikiadmin talk contribs created page Viral transduction of mammalian cells via spinfection (Created page with "{| class="wikitable" |+ !Materials ! |- | |Virus containing supernatant |- | |1000x Polybrene (Hexadimethrine bromide) in PBS (8mg/ml) 1*106 suspension cells or 1*105 adherent cells |- | |24 well plate (for suspension cells) 6 well plate (for adherent cells) |} === Procedure === ---- # 1 ml medium containing 1*105 or1*106 cells are added into 6 or 24 well plate. # Add 2 µl 1000x Polybrene # Add 1ml of virus supernatant or a corresponding dilution of concentrated virus...") Tag: Visual edit