Coomassie Blue staining of gels

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Revision as of 14:15, 27 February 2025 by ESO wikiadmin (talk | contribs) (Created page with "{| class="wikitable" |+ !Materials ! |- |Staining Solution |25% (v/v) isopropanol 10% (v/v) acetic acid 0.03% (w/v) Coomassie Brillant Blue R250 |- |Destaining Sol. |500 mL methanol; 500 mL dH<sub>2</sub>O 100 mL acetic acid |} === Procedure === ----The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a cle...")
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Materials
Staining Solution 25% (v/v) isopropanol

10% (v/v) acetic acid 0.03% (w/v) Coomassie Brillant Blue R250

Destaining Sol. 500 mL methanol; 500 mL dH2O

100 mL acetic acid

Procedure

The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a clear background.

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