User contributions for ESO wikiadmin

17:5817:58, 29 May 2025 diff hist +1,463 Agarose gel electrophoresis No edit summary Tag: Visual edit
17:5817:58, 29 May 2025 diff hist −158 Agarose gel electrophoresis Blanked the page Tags: Blanking Visual edit
17:5817:58, 29 May 2025 diff hist −108 Template:Protokoll-Mix No edit summary
17:5617:56, 29 May 2025 diff hist −385 Template:Protokoll-Mix No edit summary
17:5617:56, 29 May 2025 diff hist +158 N Agarose gel electrophoresis Created page with "{{Protokoll-Mix |titel=PCR Reaction mix (Pipett scheme) |TAE buffer (1x) 4.84 g Tris-Base, 1 mM EDTA, 1.14 mL acetic acid, fill 1 L with A.bidest. Agarose }}"
17:5417:54, 29 May 2025 diff hist +998 N Template:Protokoll-Mix Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> <div>{{{titel|Reaction mix}}}</div> </div> <div style="padding: 12px 15px; color: #333; font-size: 13px; font-family: monospace;"> <div> {{{..."
17:5317:53, 29 May 2025 diff hist +99 Template:Minimal-Box No edit summary current
17:4817:48, 29 May 2025 diff hist +567 MediaWiki:Common.css No edit summary
17:4617:46, 29 May 2025 diff hist +842 MediaWiki:Common.css No edit summary
17:4617:46, 29 May 2025 diff hist −1,006 MediaWiki:Common.css No edit summary
17:3917:39, 29 May 2025 diff hist +16 Colony PCR No edit summary current Tag: Visual edit
17:3817:38, 29 May 2025 diff hist +120 Colony PCR No edit summary
17:3117:31, 29 May 2025 diff hist +467 N Template:Minimal-Box Created page with "<div style="display: inline-block; border: 1px solid #a2a9b1; border-radius: 2px; margin: 10px 0; max-width: fit-content; background: #ffffff; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <div style="background: #eaecf0; padding: 8px 12px; border-bottom: 1px solid #a2a9b1; font-weight: bold; color: #333; font-size: 14px;"> {{{titel|Titel}}} </div> <div style="padding: 12px 15px; color: #333; font-size: 13px;"> {{{inhalt|Inhalt der Box}}} </div> </div>"
16:5316:53, 29 May 2025 diff hist +420 MediaWiki:Common.css No edit summary
16:5216:52, 29 May 2025 diff hist +214 Template:Box No edit summary current
16:5216:52, 29 May 2025 diff hist +84 Template:Labor-Boxes No edit summary
16:5016:50, 29 May 2025 diff hist −790 AG Hauck - LabWiki No edit summary
16:4216:42, 29 May 2025 diff hist +872 N Template:Protokoll-Box Created page with "<div style="border: 1px solid #ddd; border-radius: 8px; padding: 20px; margin: 20px 0; background: #fff;"> <h2 style="color: #0645ad; border-bottom: 2px solid #0645ad; padding-bottom: 10px;"> 🧪 {{{titel|Laborprotokoll}}} </h2> <div style="display: grid; grid-template-columns: 2fr 1fr; gap: 20px; margin-top: 15px;">  <div> <h3>Beschreibung</h3> <p>{{{beschreibung|Protokollbeschreibung hier}}}</p> <h3>Mat..." current
16:4216:42, 29 May 2025 diff hist +700 N Template:File-Preview Created page with "<div style="border: 1px solid #ddd; border-radius: 4px; padding: 10px; margin: 10px 0; background: #f9f9f9; display: inline-block; width: 200px;"> <div style="text-align: center; margin-bottom: 8px;"> <div style="width: 64px; height: 64px; margin: 0 auto; background: {{{color|#d73027}}}; border-radius: 4px; display: flex; align-items: center; justify-content: center; color: white; font-weight: bold; font-size: 18px;"> {{{icon|📄}}} </div> </div> <di..." current
16:3916:39, 29 May 2025 diff hist +243 Template:Labor-Boxes No edit summary
16:3116:31, 29 May 2025 diff hist +54 Template:Labor-Boxes No edit summary
16:2416:24, 29 May 2025 diff hist +2,192 N Template:Labor-Boxes Created page with "<div style="display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin: 20px 0;">  <div style="background: #e8f4fd; border: 1px solid #3366cc; border-radius: 4px; padding: 16px; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> <h3 style="margin-top: 0; color: #0645ad; border-bottom: 1px solid #3366cc; padding-bottom: 8px; margin-bottom: 12px;">🧪 Protokolle</h3> <div>Dokumentation aller Laborexperimente und Versuchsabläufe.</div> <div st..."
16:2116:21, 29 May 2025 diff hist +1,174 N Template:Homepage-Grid Created page with "<div style="display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin: 20px 0;"> <div style="grid-column: 1;"> {{Colored-Box |title={{{title1|Titel 1}}} |content={{{content1|Inhalt Box 1}}} |background={{{bg1|#e8f4fd}}} |border={{{border1|#3366cc}}} |link={{{link1|}}} |link-text={{{linktext1|Mehr →}}} }} </div> <div style="grid-column: 2;"> {{Colored-Box |title={{{title2|Titel 2}}} |content={{{content2|Inhalt Box..." current
16:2016:20, 29 May 2025 diff hist +707 N Template:Colored-Box Created page with "<div class="colored-box" style="background: {{{background|#f8f9fa}}}; border: 1px solid {{{border|#a2a9b1}}}; border-radius: 4px; padding: 16px; margin: 10px 0; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> {{#if:{{{title|}}}|<h3 style="margin-top: 0; color: {{{title-color|#0645ad}}}; border-bottom: 1px solid {{{border|#a2a9b1}}}; padding-bottom: 8px; margin-bottom: 12px;">{{{title}}}</h3>}} <div style="color: {{{text-color|#333}}};"> {{{content|Box-Inhalt hier}}} <..." current
16:1816:18, 29 May 2025 diff hist +1,174 N Vorlage:Homepage-Grid Created page with "<div style="display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin: 20px 0;"> <div style="grid-column: 1;"> {{Colored-Box |title={{{title1|Titel 1}}} |content={{{content1|Inhalt Box 1}}} |background={{{bg1|#e8f4fd}}} |border={{{border1|#3366cc}}} |link={{{link1|}}} |link-text={{{linktext1|Mehr →}}} }} </div> <div style="grid-column: 2;"> {{Colored-Box |title={{{title2|Titel 2}}} |content={{{content2|Inhalt Box..." current
16:1816:18, 29 May 2025 diff hist +707 N Vorlage:Colored-Box Created page with "<div class="colored-box" style="background: {{{background|#f8f9fa}}}; border: 1px solid {{{border|#a2a9b1}}}; border-radius: 4px; padding: 16px; margin: 10px 0; box-shadow: 0 1px 3px rgba(0,0,0,0.1);"> {{#if:{{{title|}}}|<h3 style="margin-top: 0; color: {{{title-color|#0645ad}}}; border-bottom: 1px solid {{{border|#a2a9b1}}}; padding-bottom: 8px; margin-bottom: 12px;">{{{title}}}</h3>}} <div style="color: {{{text-color|#333}}};"> {{{content|Box-Inhalt hier}}} <..." current
16:1516:15, 29 May 2025 diff hist +170 N Vorlage:Tip Created page with "<div style="border-left: 4px solid #8b5cf6; background: #f5f3ff; padding: 12px; margin: 16px 0; border-radius: 2px;"> <strong>💡 Tipp:</strong> {{{1|Tipp-Text}}} </div>" current
16:1416:14, 29 May 2025 diff hist +179 N Vorlage:Info Created page with "<div style="border-left: 4px solid #00af89; background: #f0fdf4; padding: 12px; margin: 16px 0; border-radius: 2px;"> <strong>ℹ️ Info:</strong> {{{1|Informationstext}}} </div>" current
16:1416:14, 29 May 2025 diff hist +174 N Vorlage:Warning Created page with "<div style="border-left: 4px solid #d73027; background: #fdf2f2; padding: 12px; margin: 16px 0; border-radius: 2px;"> <strong>⚠️ Warning:</strong> {{{1|Warntext}}} </div>" current
16:1316:13, 29 May 2025 diff hist +72 Vorlage:Note Hinweisbox neu designt current
16:1216:12, 29 May 2025 diff hist +1,013 MediaWiki:Common.css No edit summary
16:1116:11, 29 May 2025 diff hist −173 Vorlage:Note No edit summary
16:0916:09, 29 May 2025 diff hist +275 N Vorlage:Note Hinweisbox
15:5615:56, 29 May 2025 diff hist +79 Template:Print-Button No edit summary current
15:5515:55, 29 May 2025 diff hist −166 Template:Print-Button No edit summary
15:5315:53, 29 May 2025 diff hist −1 Template:Print-Button No edit summary
15:5215:52, 29 May 2025 diff hist −10 Template:Print-Button No edit summary
15:5115:51, 29 May 2025 diff hist −129 Template:Print-Button No edit summary
15:5015:50, 29 May 2025 diff hist −721 Template:Print-Button No edit summary
15:4915:49, 29 May 2025 diff hist +121 Template:Print-Button No edit summary
15:4715:47, 29 May 2025 diff hist +1,004 N Template:Print-Button Created page with "<div class="print-export-buttons" style="float: right; margin: 10px 0; padding: 10px; border: 1px solid #ddd; background: #f8f9fa; border-radius: 3px;"> <strong>📄 Export:</strong><br> <button onclick="window.print()" style="padding: 5px 10px; margin: 2px; background: #0645ad; color: white; border: none; cursor: pointer; border-radius: 3px;"> 🖨️ Drucken/PDF </button><br> <button onclick="copyContent()" style="padding: 5px 10px; margin: 2px; background:..."
15:4515:45, 29 May 2025 diff hist +28 Cultivation of HEK293T cells No edit summary current Tag: Visual edit
15:4515:45, 29 May 2025 diff hist +394 MediaWiki:Common.css No edit summary
15:4415:44, 29 May 2025 diff hist +1,004 N Vorlage:Print-Button Print/Export-Button-Vorlage erstellt current
15:1015:10, 29 May 2025 diff hist +20 Colony PCR No edit summary Tag: Visual edit
15:1015:10, 29 May 2025 diff hist +1,484 N Colony PCR Created page with "Reaction mix: H<sub>2</sub>O 16.5 μL 10x Taq/Pfu buffer 2 μL Primer 1 0.4 μL (10 pmol) Primer 2 0.4 μL (10 pmol) dNTP-Mix 0.2 μL Taq polymerase 0.5 μL total ..." Tag: Visual edit

14:1414:14, 21 May 2025 diff hist +1,054 N Restriction digest of plasmid DNA Created page with "{| class="wikitable" |+ !Materials |- |Restriction enzyme stock (–20°C) |- |Appropriate 10x enzyme buffer (–20°C) |- |Sterile dd H<sub>2</sub>O |- |Depending on the digestion '''(optional)''': '''''BSA (bovine serum albumin)''''': To ensure 100% activity of the enzymes, 100 µg/ml BSA should be added (stored at –20°C) |} == Procedure for analysis purposes == * 300 ng of DNA samples (e.g. control digest of plasmid minipreps) * 1 µl appropriate enzyme buffer (1..." current Tag: Visual edit
14:1114:11, 21 May 2025 diff hist +1,326 N Plasmid-Miniprep (Birnboim – Dooley protocol) Created page with "{| class="wikitable" |+ !Materials |- |Buffer P1: Solve 6.055 g Tris Base and 3.722g EDTA x 2H<sub>2</sub>O in 800 ml H<sub>2</sub>0. Adjust pH to 8.0 with 1M HCl, fill up to 1L. Per 1 ml buffer P1, add 100 µg RNase A. Store at 4°C. |- |Buffer P2: Solve 8.0 g NaOH in 950 ml H<sub>2</sub>0. Add 50 ml 20% SDS. Store at RT. |- |Puffer P3: 294.45g KAc in 500 ml H<sub>2</sub>O. Adjust pH to 5.5 with glacial acetic acid (about 110 ml acid needed), fill up to 1L with H<sub>..." Tag: Visual edit

15:2715:27, 20 May 2025 diff hist +2,298 N Vorlage Klonierungsprojekt Created page with "<noinclude> == Vorlage für Klonierungsprojekte == Diese Seite dient als Vorlage für neue gentechnische Arbeiten im Labor (z. B. PCR-Klonierungen). Kopiere den folgenden Inhalt in eine neue Wiki-Seite und passe ihn projektbezogen an. '''Hinweis:''' Alle Datumsangaben, Enzyme, Vektoren etc. bitte entsprechend aktualisieren.</noinclude> == Projekttitel == ''(z. B. Klonierung von GFP-tagged PAK1 in pcDNA3.1)'' * Startdatum: __.__.20__ * Bearbeiter: Benutzer:DeinN..." current Tag: Visual edit
15:2715:27, 20 May 2025 diff hist +47 Flow chart of a PCR-based cloning project No edit summary current Tag: Visual edit

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