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• 12:43, 5 June 2025 ESO wikiadmin talk contribs created page Immunoprecipitation (Created page with "This is an original excerpt from MHE's digital labbook from 25 April 2025. {{Protokoll-Box}} = Immunoprecipitation = -       THP-1 Cells (WT, PPM1F KO (clone D7), PPM1F KO + PPM1F WT (clone C9), PPM1F KO+ PPM1F D360A (clone A5)) were count and 3x10⁶ cells were lysed using 1000 µl RIPA lysis buffer (10 µg/ml Aprotinin,10 µM Benzamidin, 5 µg/ml Leupepin, 1 µM PMSF, 10 nM pNPP, 2 µM cytochalasinD, cpd26, 1x Phosstop) -       Cells were vortexted, s...") Tag: Visual edit