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20 May 2025
- 15:0315:03, 20 May 2025 Bacterial invasion assay by flow cytometry (hist | edit) [1,704 bytes] ESO wikiadmin (talk | contribs) (Created page with "{| class="wikitable" |+ !Materials (for 6 cm dishes) |- |CFSE |- |FACS buffer (1% (F)CS in PBS, 50-100 mL (ice-cold) |- |Trypsin/EDTA (1mL/dish) |- |Medium with serum according to cell type (3mL/dish) |- |15 mL PP falcon tubes |- |Trypan blue solution, 0.4 mg/mL |- |Eppendorf tubes 1.5mL (1 per dish) |} === Procedure === Bacteria: * 2 days before the experiment, streak bacteria from glycerol stock on selective GC or TSB agar plates (with antibiotics when needed) and in...") Tag: Visual edit
- 09:5709:57, 20 May 2025 Staining of cells for flow cytometry (hist | edit) [1,663 bytes] ESO wikiadmin (talk | contribs) (Created page with "{| class="wikitable" |+ ! ! ! ! |- |FACS buffer: |1xPBS FCS (heat-inactivated, final concentration: 5%) 5% Natriumazid (final concentration: 0.1%) | | |- |1st antibody: |primary antibody (approx. dilution 1:200) | | |- |2nd antibody: |secondary antibody (approx. dilution 1:500) fluorescence- labelled | | |- |controls: |sample with irrelevant primary antibody and secondary antibody sample with secondary antibody only if possible untransfected cells stained with both antib...") Tag: Visual edit
- 09:5409:54, 20 May 2025 Scanning electron microscopy (SEM) (hist | edit) [2,243 bytes] ESO wikiadmin (talk | contribs) (Created page with "{| class="wikitable" |+ !Materials |- |'''Fixation buffer''': 3% Formaldehyde, 2% Glutardialdehyde, 10 mM CaCl<sub>2</sub>, 10 mM MgCl<sub>2</sub>, 90 mM Sucrose in 100 mM HEPES, pH 7.2 |- |'''Washing Buffer''': 10 mM CaCl<sub>2</sub>, 10 mM MgCl<sub>2</sub>, 90 mM Sucrose in 100 mM HEPES, pH 7.2 |- |'''HEPES buffer:''' 100 mM, pH 7.2 |- |'''Graded ethanol series:''' 30%, 50%, 70%, 90%, 100% ethanol p.a. |} === Procedure: === '''1A. ''Organs''''' of infected animals are...") Tag: Visual edit
- 09:4309:43, 20 May 2025 OPTIC (Opa protein triggered integrin clustering) (hist | edit) [2,588 bytes] ESO wikiadmin (talk | contribs) (Created page with "{| class="wikitable" |+ !Materials |- |1x PBS |- |Trypsin/EDTA |- |GC-Agar plates with CAM/ERM and without antibiotics Coverslips (round, 12 mm) |- |24 well plate |- |HEK culture medium (DMEM + 10% CS) Suspension medium (DMEM + 0.25% BSA) 4% paraformaldehyde (PFA) in PBS |- |Permeabilization solution (0.4% Triton-X-100 in PBS) Blocking solution (10% heat inactivated CS in PBS) Mounting medium |} === Procedure === ==== '''Day1:''' ==== ==== Seed HEK293T cells 1:40 in 6...") Tag: Visual edit
- 09:3909:39, 20 May 2025 Live cell microscopy (hist | edit) [1,282 bytes] ESO wikiadmin (talk | contribs) (Created page with "=== Preparation of culture dishes === * In 3.5 cm culture dishes (Sarstedt 83.1800 35x10 mm) a hole is drilled with 14 mm in diameter (will be done by the uni garage) * Before sticking the coverslips (Menzel 24 mm, Stärke 1,5; CB00240RAC20MNZ#0) to the dishes spray them with 70% ethanol and wipe with a KimWipe tissue * The coverslips are pretreated as following: ** Wash with a mixture of 50% v/v Aceton and 50% v/v EtOH ** Wash with 1xPBS ** Wash with MilliQ water ** Dr...") Tag: Visual edit
7 May 2025
- 15:4515:45, 7 May 2025 Lab Etiquette (ENG) (hist | edit) [19,216 bytes] ESO wikiadmin (talk | contribs) (Created page with "'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;"> '''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>''' A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let p...")
- 15:2815:28, 7 May 2025 Laborbuchführung (hist | edit) [3,248 bytes] ESO wikiadmin (talk | contribs) (Created page with "'''Diese Seite ist auch auf Englisch verfügbar.''' = Laboratory Bookkeeping Guide = == Ein praxisorientierter Leitfaden für sauberes und effizientes Arbeiten im Forschungslabor == === Warum Laborbuchführung wichtig ist === Saubere Buchführung im Labor ist kein lästiger Formalismus – sie ist ein Grundpfeiler wissenschaftlicher Integrität. [...] === Grundprinzipien === # '''Nachvollziehbarkeit''' # '''Unveränderbarkeit''' # '''Z...")
- 15:2715:27, 7 May 2025 Laboratory Bookkeeping Guide (hist | edit) [2,796 bytes] ESO wikiadmin (talk | contribs) (Created page with "'''This page is also available in German.''' = Laboratory Bookkeeping Guide = == A practical guide to structured and efficient lab documentation == === Why lab bookkeeping matters === Clean lab documentation isn’t just bureaucracy – it’s a cornerstone of scientific integrity. [...] === Core principles === # '''Traceability''' # '''Immutability''' # '''Timeliness''' # '''Structure''' === Analog or digital? === [...] === What to include =...") Tag: Visual edit