Protein Phosphatase Assay: Revision history

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27 February 2025

  • curprev 13:0613:06, 27 February 2025 ESO wikiadmin talk contribs 3,362 bytes +3,362 Created page with "{| class="wikitable" |+ !Materials ! |- | |black flat-bottom 384-well plates (Greiner Bio-One, Germany) |- | |phosphatase buffer (50 mM TrisHCl, pH = 8.0 at 25°C, 200 mM NaCl, 20 mM MnCl2, 2 mM TCEP, 0.01% Tween). TCEP and Mn<sup>2+</sup> are added freshly; 1L bottle is sterile filtered and stored at 4°C. |- | |4-Methylumbelliferyl phosphate (4-MUP) (Sigma Aldrich, Germany) or DiFMUP (ABCR GmbH) is dissolved in DMSO (10 mM stock solution) and stored at -50°C |- | |rec..." Tag: Visual edit
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