Coomassie Blue staining of gels: Revision history

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27 February 2025

  • curprev 14:1514:15, 27 February 2025 ESO wikiadmin talk contribs 489 bytes +489 Created page with "{| class="wikitable" |+ !Materials ! |- |Staining Solution |25% (v/v) isopropanol 10% (v/v) acetic acid 0.03% (w/v) Coomassie Brillant Blue R250 |- |Destaining Sol. |500 mL methanol; 500 mL dH<sub>2</sub>O 100 mL acetic acid |} === Procedure === ----The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a cle..." Tag: Visual edit
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