View source for Coomassie Blue staining of gels

{| class="wikitable"
|+
!Materials
!
|-
|Staining Solution
|25% (v/v) isopropanol
10% (v/v) acetic acid
0.03% (w/v) Coomassie Brillant Blue R250
|-
|Destaining Sol.
|500 mL methanol; 500 mL dH<sub>2</sub>O
100 mL acetic acid
|}

=== Procedure ===
----The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a clear background.