Main public logs

Combined display of all available logs of Hauck LabWiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 14:10, 27 February 2025 ESO wikiadmin talk contribs created page Whole cell lysates (WCLs) of eukaryotic cells (Created page with "{| class="wikitable" |+ !Materials ! |- | |PBS 1X |- | |RIPA buffer |- | |Sepharose Beads |} === Procedure === ----All following steps are performed in the cold room: * Aspirate cell culture medium with a pasteur pipette * Rince attaching cells carefully with cold PBS * Add 1 mL RIPA buffer per 10 cm dish or 0.5 mL per 6 cm dish and incubate for approx. 2 min at 4°C * Carefully detach the cells with a cell scraper and transfer the lysate in a fresh tube * Mechanical s...") Tag: Visual edit