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• 18:48, 15 February 2025 ESO wikiadmin talk contribs created page Transduction and titration of lentiviral supernatant (Created page with "{| class="wikitable" |+ !Materials ! |- | |DMEM + 10% CS |- | |Puromycin (stock solution 5 mg/ml) |} === Procedure === # 1x10⁴ 293T cells are seeded in a 24-well plate ~12 h before transduction (5 wells) # 1µl, 2µl or 5µl, respectively, of the virus-concentrate are added on top of the cells, 2 wells remain untreated # incubation over night at 37°C and 5% CO₂ # 24 h after transduction the medium is replaced by 1 ml fresh medium containing 10% C...") Tag: Visual edit