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• 15:06, 20 May 2025 ESO wikiadmin talk contribs created page Tissue/embryo preparation fo cryosectioning (Created page with "{| class="wikitable" |+ !Materials |- |PBS pH 7.4 |- |4% PFA in PBS |- |10%, 20% and 30% sucrose solution in PBS |- |OCT Tissue-Tek |- |Histology moulds |- |Pre-coated glass slides |- |Dry ice |} === Procedure: === * Tissues/ anesthetized embryos are dissected and placed into 4% PFA solution overnight at 4° C * The next day the specimen is washed with PBS and transferred to a 10% sucrose solution for 3 h at 4° C (keep at 4°C until specimen sinks) * The sample is tra...") Tag: Visual edit