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- 13:31, 27 February 2025 ESO wikiadmin talk contribs created page StrepTactin-Pulldown (Created page with "{| class="wikitable" |+ !Materials ! |- |Recombinant protein |Bait protein TwinStrepTagII tagged protein, target proteins (HisSUMO or GST tagged protein of interest) |- |Pull down buffer |50 mM Tris/Cl pH 8, 150 mM NaCl, 10% Glycerol, 0.05% Tween. Optional: 10 µM ZnCl<sub>2</sub> in case of Zinc- finger proteins or 2 mM MgCl<sub>2</sub>, CaCl<sub>2</sub> or MnCl<sub>2</sub> in case of metalloproteins) |- |Elution buffer EXT |50 mM Tris/Cl pH 8, 150 mM NaCl, 50 mM Biotin...") Tag: Visual edit