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• 13:27, 27 February 2025 ESO wikiadmin talk contribs created page Rac pulldown assay (with GST-PBD beads) (Created page with "{| class="wikitable" |+ !Materials ! !''add fresh'' |- |Lysis Buffer |Tris pH 7.5, 50 mM, NaCl 150 mM, MgCl2 10 mM, NP40 1%, Glycerol 10% | rowspan="2" |DTT 1 mM, Leupeptin, Aprotinin, PMSF |- |Wash Buffer |Tris pH 7.5, 25 mM, NaCl 40 mM, MgCl₂ 30 mM, NP40 1% |- | |Glutathione-Sepharose beads with bound recombinant GST-PAK-binding-domain (GST-PBD) | |} === Procedure === ---- * Prepare lysis and wash buffer and keep on ice. Thaw beads (-80°C) on ice. * Stimu...") Tag: Visual edit