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• 14:27, 5 June 2025 ESO wikiadmin talk contribs created page Protein expression and purification (Created page with "== '''Expression of  HisSUMO-hPOPX2 (SAK) – Part 1/2''' == -      '''''Bacteria Sample'':''' #570 BL21 DE3 pRos. pET24a His-SUMO_hPopx2_fulllength -      One day prior, aprox. 15:00 : inoculate 50 mL of antibiotics containing media with 3 colonies and incubate o/n at 37°C -      7 mL each of overnight culture were used to inoculate 3 x 333 mL of antibiotics (Kan, cam) containing LB-medium in a 1 L Erlenmeyer flask until OD = 0.7 -      When OD is reach...") Tag: Visual edit