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• 13:50, 27 February 2025 ESO wikiadmin talk contribs created page Preparation of deterg.-resist. membrane microdomains (Sucrose Gradient) (Created page with "{| class="wikitable" |+ |'''Materials''' | colspan="3" | |- |Homogenisation buffer |50 mM Tris pH 7.4 |[0.5M] |▶ 10 ml |- | |2 mM MgCl₂ 8 % sucrose |[1M] |▶ 200 µl ▶8 g |- | |ddH₂O | |▶ 89.8 ml |- |TNE buffer | colspan="2" |20 mM TrisHCL pH8 [1M] |▶ 2 ml |- | | colspan="2" |130 mM NaCl [5M] |▶ 2.6 ml |- | | colspan="2" |5 mM EDTA [0.5M] |▶ 1 ml |- | | colspan="2" |10 µg/ml Aprotinin [5mg/ml] |▶ 200 µl |- | | colspan="2" |10 µg/...") Tag: Visual edit