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• 13:38, 27 February 2025 ESO wikiadmin talk contribs created page Luciferase Reporter Assay (Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Ice bath for buffers, Luciferin and lysates PBS rubber policeman (cold room) and Eppis for lysates, Eppi centrifuge at RT Lysis buffer (1M DTT → 2 µl per ml) (fridge 4°C) Assay buffer (1M DTT → 10 µl per 10 ml; 200 mM ATP → 100ul per 10 ml) (fridge 4°C) Luciferin aliquots (-20°C freezer) Multipipette and plastic box for solution (cell culture room) | | |- |Assay buffer (100ml): |25 mM Tris pH 7.8 [1 M] → 2.5 ml...") Tag: Visual edit