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• 12:04, 30 May 2025 ESO wikiadmin talk contribs created page LIC Cloning (Ligation independent cloning) (Created page with " == Procedure == Design primers compatible for cloning in vector containing the LIC sequence (e.g. pDNR Dual LIC) and perform PCR. Separate 4 µl of PCR reaction via agarose gel electrophoresis to check PCR product. Many unspecific bands besides the desired one require gel separation of the PCR sample and extraction of the desired band. If necessary, 2-3 PCR reactions can be pooled to gain more material. Add 10x NEB2 Buffer to the PCR sample to reach 1x end concentration...") Tag: Visual edit: Switched