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• 18:06, 15 February 2025 ESO wikiadmin talk contribs created page Isolation of human granulocytes (Created page with "{| class="wikitable" !Materials ! |- | |Biocoll |- | |3.8% citrate solution PBS |- | |PVA 7200 polyvinyl alcohol 2x PBS, pH 7.4 |- | |Bidest H₂O |- | |Phagocyte buffer (PBS, 0.9 mM CaCl₂; 0.5 mM MgCl₂; 5 mM glucose; 1% h.i FCS) |} === Preparations === PVA1% PVA 7200 + 0.9% NaCl * Add 5 g PVA to 500 ml 0.9 % NaCl solution * Solve with stirring on a heating plate * Autoclave * Cool under stirring * Store on shelf, protected from light =...") Tag: Visual edit