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- 10:47, 7 March 2025 ESO wikiadmin talk contribs created page Immunofluorescence staining (Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |PBS++ (50 ml PBS; 25 μl 2.5M CaCl<sub>2</sub> ; 1M 50 μl MgCl<sub>2</sub>) | | |- | |Permeabilization solution (PBS++; 10% FCS; 0.2% Saponin; alternatively 0.2% | | |- | |Triton X-100 may be used instead of saponin as a detergent) | | |- | |Blocking solution (PBS++; 10% FCS) | | |- | |First and second antibody | | |- | |Mounting medium | | |- | |Nail polish | | |} === Procedure === ---- * After fixation wash (3 x with PB...") Tag: Visual edit