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• 18:52, 15 February 2025 ESO wikiadmin talk contribs created page Concentration of lentiviral particles via ultracentrifugation (Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Virus containing supernatant | | |- | |20% sucrose in PBS | | |- | |1% BSA in PBS | | |} === Procedure === ---- # 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant # the tubes are placed in aerosol tight swing buckets # centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti) # after discarding the supernatant the pellet is...") Tag: Visual edit