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• 15:30, 30 May 2025 ESO wikiadmin talk contribs created page Cloning of guideRNA-Oligo into pBluescript U6-MCS (Created page with "== Procedure == '''gRNA Oligo annealing''' * annealing reaction contains: 1 µl sense primer (stock solution 100 µM), 1 µl antisense primer (stock solution 100 µM), 5 µl NEB buffer 2 and 43 µl ddH₂O * Annealing reaction occurs in thermocycler with the corresponding program: {| class="wikitable" |95°C |4 minutes |- | colspan="2" |Start loop, 54x, 94°C, 1 min (-1°C/loop) |- |8°C |Store forever |} == Preparation of BbsI digested vector pBluescript_U6...") Tag: Visual edit