Cloning of shRNA into lentiviral vector pLKO.1

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Procedure

Primer annealing

  • annealing reaction contains: 1 µl sense primer (stock solution 100 µM), 1 µl antisense primer (stock solution 100 µM), 5 µl NEB buffer 2 and 43 µl ddH2O
  • Annealing reaction occurs in thermocycler with the corresponding program:
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