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A user with 403 edits. Account created on 13 February 2025.
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3 June 2025

2 June 2025

  • 09:2809:28, 2 June 2025 diff hist +1,326 N FBA-staining of bacteria Created page with "{{Protokoll-Mix|Inhalt=(5-(6)-carboxyfluorescein succinimidyl ester (CFSE, FAM SE, Fluorescein)<br>NHS-Biotin<br>PBS++ (PBS, 45 nM CaCl 2, 35 nM MgCl 2)<br>4% Paraformaldehyde (PFA)<br>Blocking solution (10% FCS in PBS ++)<br>PBS<br>Streptavidin-AlexaFluor647}} == Procedure == * Dilute 0.2 mg NHS-Biotin in 1 ml PBS (prewarm Biotin to RT before opening the tube) * Collect bacteria from plate in PBS, centrifuge for 4 min, 4000 rpm, and resuspend the pellet in 700 l PB..." current Tag: Visual edit
  • 09:0709:07, 2 June 2025 diff hist +138 Gentamicin Protection Assay No edit summary current Tag: Visual edit
  • 09:0409:04, 2 June 2025 diff hist +1,553 N Gentamicin Protection Assay Created page with "== Procedure == * Aspirate cell culture medium with a pasteur pipette * Rince attaching cells carefully with cold PBS, aspirate PBS * Add 1.5 mL Trypsin/EDTA and study the detachment of the cells in the microscope * Inactivate Trypsin/EDTA with 3.5 mL medium, transfer the cell suspension into a 15 mL centrifuge tube * Pipette 20 mL of the cell suspension into a Neubauer counting chamber * Centrifuge the remaining cells at 600 rpm for 3 min * During centrifugation: Deter..." Tag: Visual edit
  • 09:0009:00, 2 June 2025 diff hist −27 Monitoring bacterial growth No edit summary current Tag: Visual edit
  • 08:5408:54, 2 June 2025 diff hist +23 Monitoring bacterial growth No edit summary Tag: Visual edit
  • 08:5308:53, 2 June 2025 diff hist +2,524 N Monitoring bacterial growth Created page with "{{Protokoll-Mix|Inhalt=Depending on the bacterium you want to analyse, you should determine beforehand: *Growth temperature *Shaking intensity *Generation time *Appropriate medium *Liquid or plate culture}} == Pre-culture == Streak out bacteria one to two days prior to the experiment: two days if you want to do a selection for single clones or if you have to incubate them on antibiotics. Addition of antibiotics usually hampers with the growth - for the determination o..." Tag: Visual edit
  • 08:4908:49, 2 June 2025 diff hist +1,000 N Transformation of Neisseria gonorrhoeae Created page with "{{Protokoll-Mix|Inhalt=Gonococcal strain which is Opa-postive /Pili positive PPM medium (15g proteose pepton, 5g NaCl, 4g KH 2PO4 1g K2HPO4, 1g soluble starch, ad 1l H20 ph: 7.5) + vitamin mix cloned and sequenced analysed gonococcal DNA which contains the spectinomycin resistance cassette and the Neisserial DNA uptake sequence (DUS) (plasmid DNA}} == Procedure == * Ngo strain (O+/P+) were grown overnight (37°C) on GC agar plates in a humid atmosphere with 5% CO 2 * N..." current Tag: Visual edit
  • 08:4208:42, 2 June 2025 diff hist +376 Transformation of plasmids in E. coli No edit summary current Tag: Visual edit
  • 08:3908:39, 2 June 2025 diff hist +890 N Transformation of plasmids in E. coli Created page with " == Procedure == * Before transformation, appropriate aliquots of competent bacteria should be thawn on ice. * Add the plasmid DNA to 100 μl competent E. coli in a snap-cap tube and mix well. * Incubate the samples 30 min on ice. * Transfer samples for 75 seconds to a 42°C water bath or heating block and quickly back on ice. * Add 1 ml LB-Medium and incubate the bacteria for 1h at 37°C (220 rpm). * Transfer the bacterial suspension from the snap-cap tube to Eppendorf..." Tag: Visual edit
  • 08:3108:31, 2 June 2025 diff hist +42 Methods and Protocols No edit summary Tag: Visual edit

30 May 2025

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