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• 18:12, 29 May 2025 ESO wikiadmin talk contribs created page PCR approach in 50 μL with hot-start and/or touch-down (preparative PCR) (Created page with "{{Protokoll-Mix|Inhalt=20 mM dNTPs 10x Taq/Pfu buffer 2 Primers, 10 μM DNA template, 100 – 200 ng/μL DNA polymerases (Pfu, Taq)}} == Procedure == As usually more than one PCR sample is run simultaneously, reaction and enzyme mix are prepared separately. The enzyme mix is added to the reaction mix after starting the PCR and after pre-heating the reaction mix to 94 ºC (hot start). A typical 50 μL approach that yields enough material for later cloning procedures...") Tag: Visual edit