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• 14:14, 21 May 2025 ESO wikiadmin talk contribs created page Restriction digest of plasmid DNA (Created page with "{| class="wikitable" |+ !Materials |- |Restriction enzyme stock (–20°C) |- |Appropriate 10x enzyme buffer (–20°C) |- |Sterile dd H₂O |- |Depending on the digestion '''(optional)''': '''''BSA (bovine serum albumin)''''': To ensure 100% activity of the enzymes, 100 µg/ml BSA should be added (stored at –20°C) |} == Procedure for analysis purposes == * 300 ng of DNA samples (e.g. control digest of plasmid minipreps) * 1 µl appropriate enzyme buffer (1...") Tag: Visual edit