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• 15:03, 20 May 2025 ESO wikiadmin talk contribs created page Bacterial invasion assay by flow cytometry (Created page with "{| class="wikitable" |+ !Materials (for 6 cm dishes) |- |CFSE |- |FACS buffer (1% (F)CS in PBS, 50-100 mL (ice-cold) |- |Trypsin/EDTA (1mL/dish) |- |Medium with serum according to cell type (3mL/dish) |- |15 mL PP falcon tubes |- |Trypan blue solution, 0.4 mg/mL |- |Eppendorf tubes 1.5mL (1 per dish) |} === Procedure === Bacteria: * 2 days before the experiment, streak bacteria from glycerol stock on selective GC or TSB agar plates (with antibiotics when needed) and in...") Tag: Visual edit