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• 09:43, 20 May 2025 ESO wikiadmin talk contribs created page OPTIC (Opa protein triggered integrin clustering) (Created page with "{| class="wikitable" |+ !Materials |- |1x PBS |- |Trypsin/EDTA |- |GC-Agar plates with CAM/ERM and without antibiotics Coverslips (round, 12 mm) |- |24 well plate |- |HEK culture medium (DMEM + 10% CS) Suspension medium (DMEM + 0.25% BSA) 4% paraformaldehyde (PFA) in PBS |- |Permeabilization solution (0.4% Triton-X-100 in PBS) Blocking solution (10% heat inactivated CS in PBS) Mounting medium |} === Procedure === ==== '''Day1:''' ==== ==== Seed HEK293T cells 1:40 in 6...") Tag: Visual edit