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• 14:44, 27 February 2025 ESO wikiadmin talk contribs created page Dot Blot (Created page with "{| class="wikitable" |+ !Materials ! |- | |Nitrocellulose membrane / Whatman paper |- | |TBS-T |- | |Ponceau S |- | |Blocking Solution |- | |First and Second Antibody |- | |ECL solution and H₂O₂ |} === Procedure === ---- * Whatman paper and membrane have to be wet  first whatman on dot blot, followed by membrane. Top of dot blot holder has to be firmly secured with screws on the lower part! * Apply 50 µl of probe with equivalent amount of prot...") Tag: Visual edit