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- 14:40, 27 February 2025 ESO wikiadmin talk contribs created page Western Blot: stripping of the membrane (Created page with "{| class="wikitable" |+ !Materials ! |- |Mild Stripping Buffer |15 g Glycin, 1 g SDS, 10 ml Tween20 in 1L ddH<sub>2</sub>O pH2.2 |- |Harsh Stripping Buffer |40 ml Stacking gel buffer (0,5M Tirs/HCl, pH6,8) 10 ml SDS 20%, 200 ml ddH<sub>2</sub>O |- | |1X PBS |- | |TBST |} == Procedure mild stripping == * Remove ECL from membrane by washing membrane briefly with ddH<sub>2</sub>O * Wash membrane 2x in Mild stripping buffer for 10 min * Wash membrane 2x in PBS for 10 min *...") Tag: Visual edit