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• 14:26, 27 February 2025 ESO wikiadmin talk contribs created page Western Blot: semi-dry blot (Created page with "{| class="wikitable" |+ !Materials ! |- |5X Anode buffer |15 g Tris Base, pH to 10.4, final volume 1l |- |5X Catode buffer |15 g Tris Base, 26.25 g 6-amino-N-caproic acid (6-Amino-hexansäure), pH 9.4; final volume 1 l |} === Procedure === ---- * Anode Buffer preparation: 20 ml 5x Anode buffer, 10 ml MeOH, fill up to 100 ml with ddH2O * Cathode Buffer preparation: 20 ml 5x Cathode buffer, 10 ml MeOH, fill up to 100 ml with ddH2O * The PVDF membrane is labeled with a pe...") Tag: Visual edit