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• 14:23, 27 February 2025 ESO wikiadmin talk contribs created page Western Blot: wet blot (Created page with "{| class="wikitable" |+ !Materials ! |- |Western Transfer Buffer |dissolve 6.0 g Tris Base; 28.8 g glycine in 1 L dH₂O add 430 mL methanol and degas for 30 min while stirring after degasing, add 10 mL 20% SDS and fill up to 2 L with H₂O |- |Blocking Solution |TBS-T; 2% BSA; 0.05% NaN₃ |- |TBS-Tween |200 mL 10x TBS; 10 mL Tween; ad 2 L H₂O |- | |First and second Antobody |- | |ECL-Medium |- | |H₂O₂ (30%) |-...") Tag: Visual edit