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- 13:08, 27 February 2025 ESO wikiadmin talk contribs created page In vitro Tyrosine Kinase Activity Assay (Created page with "{| class="wikitable" |+ !Materials ! |- | |Kinase buffer (125 mM NaCl; 48 mM MgCl<sub>2</sub>; 50 mM HEPES (pH 7.5) |- | |4x SDS Buffer |- | |1 mM ATP |- | |Purified Kinase (e.g. Src or FAK) |- | |Kinase substrate (e.g. CEACAM3-cytoplasmic domain or FAK substrate) |} === Procedure === ---- * 0.2 μg/ml FAK-substrate or 25 ng/μl TwinStrep-Ceacam3-cytoplasmic domains * 0.2 μg/ml hFAK KD (recombinant human FAK kinase domain) or 10 ng/μl Src * with or without (control)...") Tag: Visual edit