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- 18:55, 15 February 2025 ESO wikiadmin talk contribs created page Production of soluble CEACAM domains in human cells (Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Plasmid DNA | | |- | |DMEM |Cell culture medium with L-glutamine and 4.5 g/l glucose (PAA Laboratories) | |- | |10% CS |calf serum (PAA Laboratories) | |- | |2xHBS |16.4 g NaCl, 11.9 g HEPES, 0.21 g Na<sub>2</sub>HPO4, solve in 1 l ddH<sub>2</sub>O, pH 7, sterile filtration | |- | |CaCl<sub>2</sub> solution |2.5 M CaCl<sub>2</sub> | |- | |OptiMEM |Serum-reduced medium with L-glutamine and HEPES (Gibco) | |} === Procedure =...") Tag: Visual edit