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• 18:46, 15 February 2025 ESO wikiadmin talk contribs created page Production of lentiviral particles (Created page with "{| class="wikitable" |+ !Material ! |- | |293T cells |- | |2x HBS-buffer |- | |2.5 M CaCl₂ |- | |25 mM Chloroquin |- | |Lentiviral vectors |} === Procedure === # A confluent plate of 293T cells is splitted 1:5 one day before transfection # The transfection reaction contains: 500 µl ddH₂O, 6.5 µg pLKO.1 (with gene or shRNA of interest), 3.5 µg pMD2.G (plasmid #1256) and 5 µg psPAX2 (plasmid #1257) # Add 500 µl 2x HBS-buffer # While vortexing t...") Tag: Visual edit