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• 18:12, 15 February 2025 ESO wikiadmin talk contribs created page Infection of granulocytes (Created page with "=== Procedure === 1x10⁶ granulocytes are infected with bacteria (MOI 20) for 15 min at 37°C under rotation. Phagocytosis is stopped by the addition of 500 µl of ice cold PBS. Samples are centrifuged at 4°C for 5 min at 200 g and washed once with PBS. After centrifugation samples are taken up in PBS or SDS-buffer.") Tag: Visual edit