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• 18:10, 15 February 2025 ESO wikiadmin talk contribs created page Granulocyte FACS phagocytosis assay (Created page with "{| class="wikitable" |+ !Material ! ! ! |- | |Freshly isolated granulocytes | | |- | |Phagocytosis buffer (PBS, 0.9 mM CaCl₂; 0.5 mM MgCl₂; 5 mM glucose; 1% heat inactivated FCS) | | |- | |5-(6)-carboxyfluorescein succinimidyl ester (FAM SE; CFSE, Fluorescein, FITC) | | |- | |Trypan Blue solution | | |} === Preparations === Two days before experiment: streak out gonococci on selective agar plates and select colony morphology one day before infectio...") Tag: Visual edit