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• 17:42, 15 February 2025 ESO wikiadmin talk contribs created page Transfection of fibroblasts (Created page with "=== Materials === Plasmid DNA OptiMEM Lipofectamin 3000 or jetPrime === Procedure === The day before transfection: seed fibroblasts on gelatine coated 24 or 6-well plate so that they are approx. 70% confluent at time of transfection (for NIH3T3 cells 2.5x10^4 for 24 well, 1.5x10^5 for 6 well plate) Change medium to remove and dead cells and debris Which transfection reagent works best for your cells has to be determined empirically: ==== '''JetPrime Protocol''' for...") Tag: Visual edit