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• 16:31, 15 February 2025 ESO wikiadmin talk contribs created page Transfection of HEK293T cells (Created page with "{| class="wikitable" !Materials ! |- | |Plasmid DNA |- | |2x HBS- buffer, pH 7.05 (correct pH is extremely critical !!) |- | |2.5 M CaCl2 (sterile) |- | |25 mM chloroquin (sterile) |} '''Procedure''' * 1 day before transfection: cells split 1:3 up to 1:5 in 10cm dishes * Next day: Set up for each 10cm dish: 500 μl ddH2O (sterile) in 14 ml PP Greiner tube * Add 5 μg (or adapted amounts) of plasmid DNA * Add 500 μl 2x HBS buffer, mix sample * Add drop-wise and duri...") Tag: Visual edit