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- 09:28, 2 June 2025 ESO wikiadmin talk contribs created page FBA-staining of bacteria (Created page with "{{Protokoll-Mix|Inhalt=(5-(6)-carboxyfluorescein succinimidyl ester (CFSE, FAM SE, Fluorescein)<br>NHS-Biotin<br>PBS++ (PBS, 45 nM CaCl 2, 35 nM MgCl 2)<br>4% Paraformaldehyde (PFA)<br>Blocking solution (10% FCS in PBS ++)<br>PBS<br>Streptavidin-AlexaFluor647}} == Procedure == * Dilute 0.2 mg NHS-Biotin in 1 ml PBS (prewarm Biotin to RT before opening the tube) * Collect bacteria from plate in PBS, centrifuge for 4 min, 4000 rpm, and resuspend the pellet in 700 l PB...") Tag: Visual edit