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• 15:43, 30 May 2025 ESO wikiadmin talk contribs created page Cre-Lox recombination of plasmids (Created page with "== Procedure == * Dilute Cre-recombinase [10 U/µl] with 1x Cre-recombinase-buffer 1:10 [1 U/µl] * Recombination reaction contains: ** 1 µl 10x Cre-recombination-buffer 6µl aqua bidest. ** 1 µl (100ng) donor vector (e.g. pDNR-dual gene X) ** 1 µl (200ng) acceptor vector (e.g. pLPS3’-EGFP) ** 2 µl Cre-recombinase * Incubation for 45 min at 37° C, meanwhile starting heating-unit * Heat inactivation for 10 min at 70° C and subsequent cooling to room temperature...") Tag: Visual edit