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- 15:40, 30 May 2025 ESO wikiadmin talk contribs created page Site directed mutagenesis (Created page with "== Procedure == * Prepare the PCR reaction mix according to the hot start procedure. Include one negative control sample without Pfu polymerase (incubate sample at the lowest annealing temperature of your gradient) * Perform gradient PCR using the following thermal cycling conditions: {| class="wikitable" !Step !Temperature °C !Time !No. of cycles |- |Initial denauturation |94 |1-3 min |1 |- |Hot Start / add enzyme |94 |pause |1 |- |Denauturation |94 |20 sec | rowspan...") Tag: Visual edit